<em>The unemployment becomes one of the problems that must resolved, one way to reduce the number of unemployed is to increase student interest in entrepreneurship. Entrepreneurial interest in students can be influenced by several factors including the family environment, entrepreneurial motivation and entrepreneurial knowledge to foster one's entrepreneurial spirit. This study aims to determine the effect of family environment, entrepreneurial motivation, and entrepreneurial knowledge on interest in entrepreneurship. This research design uses explanatory research. This type of research data is quantitative. The population in this study were students of the Faculty of Economics and Business, University of Muhammadiyah Jember 2017 with a total sample of 175 respondents. The sampling technique was random sampling. This study uses Multiple Linear Regression Analysis based on SPSS 23. The results of the study that the family environment variable does not have a positive and significant effect on students' interest in entrepreneurship. Meanwhile, the entrepreneurial motivation variables and entrepreneurial knowledge have a positive and significant effect on students' entrepreneurial interest.</em>