<p>This research is entitled THE INFLUENCE OF TRAINING, ORGANIZATIONAL CULTURE, AND WORK MOTIVATION ON EMPLOYEE EFFECTIVENESS AND ITS IMPACT ON EMPLOYEE PERFORMANCE IN BANJAR CITY ONE-STOP SINGLE SYSTEM. Banjar City One-Stop Single System, is an administrative system formed to facilitate and accelerate the service of public interests whose activities are carried out in one building, the problems faced are still low employee performance influenced by lack of training, weak organizational culture and low work motivation.</p><p>This study aims to find out and analyze the influence of training, organizational culture and work motivation on work effectiveness and its impact on the performance of Banjar City Samsat employees. Therefore this study uses a quantitative approach with i SPSS program (Statistical Product and Service Solutions) Version 16.0 for Windows.</p><p>Based on the calculation results obtained by the number R2 (R Square), the influence of training, organizational culture and work motivation on the effectiveness of the Banjar City Samsat workforce is 66.5%, the effect of work effectiveness on employee performance is 82.6%, the effect of training on work effectiveness employees at 20.34%, the influence of organizational culture on employee work effectiveness of 12.75%. the effect of work motivation on employee work effectiveness of 2.43% Based on the results of the hypothesis test there is the effect of training, organizational culture and work motivation on the effectiveness of the work of Banjar City Samsat employees either partially or simultaneously.</p>